7| Cut a piece of nylon or nitrocellulose membrane and two sheets of thick blotting paper ∼1 mm larger than the gel in each dimension. Cut a corner from the membrane to match the gel (step 5).

Following separation, the proteins are transferred from the gel onto a blotting membrane. Although this step is what gives the technique the name "western blotting," the term is typically used to ...

Download this handbook to explore the most recent advances in protein blotting techniques, from membrane selection to blot visualization and beyond.

These buffers target dye-labeled antibody interactions with both non-specific proteins and the blotting membrane (either PVDF or nitrocellulose) itself, and suppress more background signal than BSA, ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types of protein ...